MCQ ON Chromatography: Chromatography is a process for separating a mixture of chemical compounds into their individual components so that the individual components can be thoroughly studied. There are many different types of chromatography, such as liquid chromatography, gas chromatography, ion-exchange chromatography, and affinity chromatography, yet they all use the same fundamental principles.
Chromatography is a technique for separation where the analyte is mixed with a liquid or gaseous mobile phase and passed through a stationary phase with a pump. Typically, there are two phases: a hydrophilic phase and a lipophilic phase. Differently with each of these two phases, the analyte’s constituents interact. They interact with the stationary phase for varying lengths of time, depending on their polarity, and are subsequently retarded to varying degrees. This causes the various components in the sample to be separated. The retention time is the time at which each component of the sample elutes from the stationary phase. A chromatogram is created as the signal from the components is recorded and plotted as they move past the detector.
MCQ on Chromatography
Q1. Chromatography is a technique that physically separates and examines __________
- Simple mixtures
- Complex mixtures
- Viscous mixtures
- Metals
Answer- Complex mixtures
Q2.In what kind of chromatography is the mobile phase pushed under pressure through a small tube containing the stationary phase?
- Column chromatography
- Planar chromatography
- Liquid chromatography
- Gas chromatography
Answer- Column chromatography
Q3. How polar is the stationary phase compared to the mobile phase in which chromatography?
- Ion exchange chromatography
- Normal phase chromatography
- Reversed chromatography
- Size exclusion chromatography
Answer- Normal phase chromatography
Q4. Which force is involved in Chromatography?
- Hydrogen bonding
- London force
- Electric static force
- All of the above
Answer- All of the above
Q5. What method is also referred to as “color writing”?
- NMR
- Mass spectroscopy
- Chromatography
- All of the above
Answer- Chromatography
Q6. Ion exchange chromatography relies on the
- Electrostatic attraction
- Electrical mobility of ionic species
- Adsorption chromatography
- Partition chromatography
Answer- Electrostatic attraction
Q7. Which of the following chromatographic techniques involves separating components in a mixture across a 0.2 mm layer of an adsorbent?
- Gas-liquid
- Column
- Thin layer
- Paper
Answer- Thin layer
Q8. Volatile chemicals cannot be purified by chromatography.
- True
- False
Answer- False
Q9. Which HPLC detector from the list below is employed as a bulk property or general detector?
- Electrochemical detector
- Fluorescence detector
- UV-Visible detector
- Evaporative Light scattering detector
Answer- Evaporative Light scattering detector
Q10. Delicate products cannot be separated using chromatography.
- True
- False
Answer- False
Q11. Which of the following approaches can be used to perform liquid chromatography?
- Only in columns
- Only on plane surfaces
- Either in columns or on plane surfaces
- Neither in columns nor on plane surfaces
Answer- Either in columns or on plane surfaces
Q12. which of the following approaches can be used to perform gas chromatography?
- Only in columns
- Only on plane surfaces
- Either in columns or on plane surfaces
- Neither in columns nor on plane surfaces
Answer- Only in columns
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Q13. A combination of paper chromatography and electrophoresis involves
- Partition chromatography
- Electrical mobility of the ionic species
- Both (a) and (b)
- None of these
Answer- Both (a) and (b)
Q14. Which of the following chromatographic methods uses a procedure where the mobile phase passes through the stationary phase under the influence of capillary action or gravity?
- Column Chromatography
- High-Pressure Liquid Chromatography
- Gas Chromatography
- Planar Chromatography
Answer- Planar Chromatography
Q15. Thin layer chromatography is__________
- Partition chromatography
- Electrical mobility of ionic species
- Adsorption chromatography
- None of the above
Answer- Adsorption chromatography
Q16. In paper chromatography, which of the following is used as a spraying reagent?
- Conc. HCl
- NaCl solution
- Ninhydrin solution
- CuSO4 solution
Answer- Ninhydrin solution
Q17. The stationary phase in reverse-phase chromatography is made
- Non-polar
- Polar
- Either non-polar or polar
- None of these
Answer- Non-polar
Q18. Column efficiency is measured in terms of a number of theoretical plates, which is:
- Inversely related to the square root of height equivalent to theoretical plates
- Directly related to the square root of height equivalent to theoretical plates
- Directly related to a height equivalent to theoretical plates
- Inversely related to a height equivalent to theoretical plates
Answer- Inversely related to a height equivalent to theoretical plates
Q19. Components with low K values have a preference towards.
______________
- Mobile phase
- Stationary phase
- No phase
- Solution
Answer- Stationary phase
Q20. The basis for the separation of the volatile material’s components in gas chromatography is the difference in
- Partition coefficients
- Conductivity
- Molecular weight
- Molarity
Answer- Partition coefficients
FAQs on MCQ on Chromatography
Chromatography is a laboratory technique used to separate and identify components of a mixture. It involves passing a mixture through a stationary phase (e.g. a column packed with solid particles) and a mobile phase (e.g. a liquid or gas) that moves the components through the stationary phase at different rates.
There are several types of chromatography, including gas chromatography (GC), high-performance liquid chromatography (HPLC), thin-layer chromatography (TLC), and paper chromatography. Each type of chromatography uses a different stationary phase and/or mobile phase to separate the components of a mixture.
MCQs on chromatography may cover a variety of topics, including the principles and theory of chromatography, types of chromatography, chromatography instrumentation, chromatography data analysis, and applications of chromatography in various fields such as chemistry, biology, and environmental science.
The separation of components in chromatography is affected by various factors such as the type of stationary and mobile phases used, the temperature and pressure of the system, and the properties of the components being separated (e.g. size, polarity, and charge).
The stationary phase is the immobile component of the chromatography system, which is typically a solid or liquid coating on a solid support. The mobile phase is the liquid or gas that carries the mixture through the stationary phase. The mobile phase moves the components through the stationary phase and separates them based on their affinity for the stationary and mobile phases.
