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MCQ on Chromatography – Check out the Top Chromatography Objective Questions Answers here!

MCQ on Chromatography

MCQ ON Chromatography: Chromatography is a process for separating a mixture of chemical compounds into their individual components so that the individual components can be thoroughly studied. There are many different types of chromatography, such as liquid chromatography, gas chromatography, ion-exchange chromatography, and affinity chromatography, yet they all use the same fundamental principles.

Chromatography is a technique for separation where the analyte is mixed with a liquid or gaseous mobile phase and passed through a stationary phase with a pump. Typically, there are two phases: a hydrophilic phase and a lipophilic phase. Differently with each of these two phases, the analyte’s constituents interact. They interact with the stationary phase for varying lengths of time, depending on their polarity, and are subsequently retarded to varying degrees. This causes the various components in the sample to be separated. The retention time is the time at which each component of the sample elutes from the stationary phase. A chromatogram is created as the signal from the components is recorded and plotted as they move past the detector.

MCQ on Chromatography

Q1. Chromatography is a technique that physically separates and examines __________

Answer- Complex mixtures

Q2.In what kind of chromatography is the mobile phase pushed under pressure through a small tube containing the stationary phase?

Answer- Column chromatography

Q3. How polar is the stationary phase compared to the mobile phase in which chromatography?

Answer-  Normal phase chromatography

Q4. Which force is involved in Chromatography?

Answer- All of the above

Q5. What method is also referred to as “color writing”?

Answer- Chromatography

Q6. Ion exchange chromatography relies on the

Answer- Electrostatic attraction

Q7. Which of the following chromatographic techniques involves separating components in a mixture across a 0.2 mm layer of an adsorbent?

Answer- Thin layer

Q8. Volatile chemicals cannot be purified by chromatography.

Answer- False

Q9. Which HPLC detector from the list below is employed as a bulk property or general detector?

Answer- Evaporative Light scattering detector

Q10. Delicate products cannot be separated using chromatography.

Answer- False

Q11. Which of the following approaches can be used to perform liquid chromatography?

Answer- Either in columns or on plane surfaces

Q12. which of the following approaches can be used to perform gas chromatography?

Answer- Only in columns

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Q13. A combination of paper chromatography and electrophoresis involves

Answer- Both (a) and (b)

Q14. Which of the following chromatographic methods uses a procedure where the mobile phase passes through the stationary phase under the influence of capillary action or gravity?

Answer- Planar Chromatography

Q15. Thin layer chromatography is__________

Answer- Adsorption chromatography

Q16. In paper chromatography, which of the following is used as a spraying reagent?

Answer- Ninhydrin solution

Q17. The stationary phase in reverse-phase chromatography is made

Answer- Non-polar

Q18. Column efficiency is measured in terms of a number of theoretical plates, which is:

Answer- Inversely related to a height equivalent to theoretical plates

Q19. Components with low K values have a preference towards.

______________

Answer- Stationary phase

Q20. The basis for the separation of the volatile material’s components in gas chromatography is the difference in

Answer- Partition coefficients

FAQs on MCQ on Chromatography

What is chromatography?

Chromatography is a laboratory technique used to separate and identify components of a mixture. It involves passing a mixture through a stationary phase (e.g. a column packed with solid particles) and a mobile phase (e.g. a liquid or gas) that moves the components through the stationary phase at different rates.

What are some common types of chromatography?

There are several types of chromatography, including gas chromatography (GC), high-performance liquid chromatography (HPLC), thin-layer chromatography (TLC), and paper chromatography. Each type of chromatography uses a different stationary phase and/or mobile phase to separate the components of a mixture.

What topics might be covered in MCQs on chromatography?

MCQs on chromatography may cover a variety of topics, including the principles and theory of chromatography, types of chromatography, chromatography instrumentation, chromatography data analysis, and applications of chromatography in various fields such as chemistry, biology, and environmental science.

What factors affect the separation of components in chromatography?

The separation of components in chromatography is affected by various factors such as the type of stationary and mobile phases used, the temperature and pressure of the system, and the properties of the components being separated (e.g. size, polarity, and charge).

What is the difference between the stationary and mobile phases in chromatography?

The stationary phase is the immobile component of the chromatography system, which is typically a solid or liquid coating on a solid support. The mobile phase is the liquid or gas that carries the mixture through the stationary phase. The mobile phase moves the components through the stationary phase and separates them based on their affinity for the stationary and mobile phases.